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How to Grow Bacteria in Agar Petri Dishes

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    • 1). Keep the agar petri dishes stored upside down in a refrigerator until you are ready to use them.

    • 2). Remove the agar petri dishes from the refrigerator and let them warm up to room temperature. It normally takes about one hour for them to warm up.

    • 3). Remove the lid of the petri dish and press the agar against any surface. If you cannot hold it directly against a surface, you can use a cotton swab. Rub the cotton swab against any surface and press it gently against the agar.

    • 4). Put the lid on the petri dish and tape it shut with masking tape. Label the tape with a marker to keep track of the bacteria source.

    • 5). Place the petri dishes in an area that has a constant temperature of roughly 100 degrees F. The dish should be away from direct sunlight, so an incubator is ideal.

    • 6). Observe the petri dishes daily. Bacteria colonies should begin to grow within a couple days.

    • 7). Pour a small amount of bleach on the petri dishes before you discard them to kill the bacteria.

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