Eriochrome Black T Solution Preparation
- 1). Put on gloves and protective eyewear and weigh out approximately 0.5 g of solid Eriochrome Black T, (EBT) on a balance and transfer it to a small beaker or flask. Add about 50 mL of 95 percent ethyl alcohol and swirl the mixture until the EBT has fully dissolved.
- 2). Weigh out 4.5 g of hydroxylamine hydrochloride on a balance and transfer it to the beaker or flask containing the EBT. Swirl until the hydroxylamine hydrochloride has fully dissolved.
- 3). Transfer the solution containing the EBT and hydroxylamine hydrochloride to a 100-mL graduated cylinder. Add enough 95 percent ethyl alcohol to bring the total volume to exactly 100 mL.
- 4). Transfer the EBT solution from the 100-mL graduated cylinder to a dropper bottle and label the bottle "0.5% Eriochrome Black T in Ethanol."